Hi Adam and welcome to GTA, nice to have you with us. As for your question.... no, you don't have to remove the barrel to tune the rifle. Unless you're having some kind of barrel issue I would just leave it alone. Good luck with that Big Cat,, I'll be looking forward to your future posts with the rifle. Also remember, if you hunt or pest control with that shooter be sure to post the stories and pics in the Hunting Gate, we just love hunting stories and pics

Jeff